MersV1, Main, Exploration, bibRecord, 000722

A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus

Identifieur interne : 000722 ( Main/Exploration ); précédent : 000721; suivant : 000723

A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus

Source :

Emerging Microbes & Infections [ 2222-1751 ] ; 2019.

RBID : PMC:4575394

Descripteurs français

KwdFr :
- Animaux, Anticorps monoclonaux (biosynthèse), Anticorps monoclonaux (immunologie), Antigènes viraux (analyse), Chameaux, Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Femelle, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (immunologie), Protéines nucléocapside (analyse), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Réservoirs d'agents pathogènes, Sensibilité et spécificité, Souris, Souris de lignée BALB C, Test ELISA (), Test ELISA (normes), Zoonoses (diagnostic), Zoonoses (immunologie).
MESH :
- analyse : Antigènes viraux, Protéines nucléocapside.
- biosynthèse : Anticorps monoclonaux.
- diagnostic : Infections à coronavirus, Zoonoses.
- génétique : Protéines nucléocapside.
- immunologie : Anticorps monoclonaux, Coronavirus du syndrome respiratoire du Moyen-Orient, Infections à coronavirus, Zoonoses.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- métabolisme : Protéines nucléocapside.
- normes : Test ELISA.
- Animaux, Chameaux, Femelle, Humains, Réservoirs d'agents pathogènes, Sensibilité et spécificité, Souris, Souris de lignée BALB C, Test ELISA.

English descriptors

KwdEn :
- Animals, Antibodies, Monoclonal (biosynthesis), Antibodies, Monoclonal (immunology), Antigens, Viral (analysis), Camelus, Coronavirus Infections (diagnosis), Coronavirus Infections (immunology), Disease Reservoirs, Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (standards), Female, Humans, Mice, Mice, Inbred BALB C, Middle East Respiratory Syndrome Coronavirus (immunology), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Nucleocapsid Proteins (analysis), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), Sensitivity and Specificity, Zoonoses (diagnosis), Zoonoses (immunology).
MESH :
- chemical , analysis : Antigens, Viral, Nucleocapsid Proteins.
- chemical , biosynthesis : Antibodies, Monoclonal.
- chemical , genetics : Nucleocapsid Proteins.
- chemical , immunology : Antibodies, Monoclonal.
- diagnosis : Coronavirus Infections, Zoonoses.
- immunology : Coronavirus Infections, Middle East Respiratory Syndrome Coronavirus, Zoonoses.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- chemical , metabolism : Nucleocapsid Proteins.
- methods : Enzyme-Linked Immunosorbent Assay.
- standards : Enzyme-Linked Immunosorbent Assay.
- Animals, Camelus, Disease Reservoirs, Female, Humans, Mice, Mice, Inbred BALB C, Sensitivity and Specificity.

Abstract

Since its emergence in 2012, more than 900 laboratory-confirmed cases of Middle East respiratory syndrome (MERS) have been reported with a fatality rate of more than 30%. However, no antigen detection assay for commercial use is available for diagnosis. In this study, the full-length nucleocapsid protein (NP) gene of MERS coronavirus (MERS-CoV) was cloned and expressed in Escherichia coli. A MERS-CoV NP capture enzyme-linked immunosorbent assay (ELISA) using two MERS-CoV-NP-specific monoclonal antibodies (MAbs) generated was developed. The ELISA was evaluated using 129 nasopharyngeal aspirates (NPAs) positive for various respiratory viruses and simulated positive NPAs by adding serial dilutions of MERS-CoV. Using a cutoff OD of 0.19, all 129 NPAs positive for respiratory viruses showed very low OD, with a specificity of 100%. For the two simulated MERS-CoV-positive NPAs with serial dilutions of live MERS-CoV, all samples with ≥10 50% tissue culture infective dose (TCID₅₀)/0.1 mL showed positive results. For the 10 additional NPAs with 20 and 200 TCID₅₀/0.1 mL of live MERS-CoV added, all were positive. A highly sensitive and specific MAbs-based antigen capture ELISA has been developed for MERS. This sensitive and specific antigen capture ELISA should be useful for detection of MERS-CoV in human and dromedaries and in field studies.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575394

DOI: 10.1038/emi.2015.26
PubMed: 26421268
PubMed Central: 4575394

Affiliations:

République populaire de Chine

Le document en format XML

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<author><name sortKey="Poon, Rosana Ws" sort="Poon, Rosana Ws" uniqKey="Poon R" first="Rosana Ws" last="Poon">Rosana Ws Poon</name>
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<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus</title>
<author><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name>
<affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University</institution>
, Xiamen 361102, Fujian Province,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University</institution>
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<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name>
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<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Kang, Yahong" sort="Kang, Yahong" uniqKey="Kang Y" first="Yahong" last="Kang">Yahong Kang</name>
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, Xiamen 361102, Fujian Province,<country>China</country>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University</institution>
, Xiamen 361102, Fujian Province,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<author><name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name>
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, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
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<affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Luk, Hayes Kh" sort="Luk, Hayes Kh" uniqKey="Luk H" first="Hayes Kh" last="Luk">Hayes Kh Luk</name>
<affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Poon, Rosana Ws" sort="Poon, Rosana Ws" uniqKey="Poon R" first="Rosana Ws" last="Poon">Rosana Ws Poon</name>
<affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chan, Jasper Fw" sort="Chan, Jasper Fw" uniqKey="Chan J" first="Jasper Fw" last="Chan">Jasper Fw Chan</name>
<affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Xia, Ningshao" sort="Xia, Ningshao" uniqKey="Xia N" first="Ningshao" last="Xia">Ningshao Xia</name>
<affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University</institution>
, Xiamen 361102, Fujian Province,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University</institution>
, Xiamen 361102, Fujian Province,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lau, Susanna Kp" sort="Lau, Susanna Kp" uniqKey="Lau S" first="Susanna Kp" last="Lau">Susanna Kp Lau</name>
<affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Woo, Patrick Cy" sort="Woo, Patrick Cy" uniqKey="Woo P" first="Patrick Cy" last="Woo">Patrick Cy Woo</name>
<affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>Department of Microbiology, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="AFF0005"><institution>Research Centre of Infection and Immunology, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="AFF0006"><institution>Carol Yu Centre for Infection, The University of Hong Kong</institution>
, Hong Kong,<country>China</country>
</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Emerging Microbes & Infections</title>
<idno type="eISSN">2222-1751</idno>
<imprint><date when="2019">2019</date>
</imprint>
</series>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Monoclonal (biosynthesis)</term>
<term>Antibodies, Monoclonal (immunology)</term>
<term>Antigens, Viral (analysis)</term>
<term>Camelus</term>
<term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (immunology)</term>
<term>Disease Reservoirs</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Enzyme-Linked Immunosorbent Assay (standards)</term>
<term>Female</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Middle East Respiratory Syndrome Coronavirus (immunology)</term>
<term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term>
<term>Nucleocapsid Proteins (analysis)</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (metabolism)</term>
<term>Sensitivity and Specificity</term>
<term>Zoonoses (diagnosis)</term>
<term>Zoonoses (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps monoclonaux (biosynthèse)</term>
<term>Anticorps monoclonaux (immunologie)</term>
<term>Antigènes viraux (analyse)</term>
<term>Chameaux</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term>
<term>Femelle</term>
<term>Humains</term>
<term>Infections à coronavirus (diagnostic)</term>
<term>Infections à coronavirus (immunologie)</term>
<term>Protéines nucléocapside (analyse)</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (métabolisme)</term>
<term>Réservoirs d'agents pathogènes</term>
<term>Sensibilité et spécificité</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Test ELISA ()</term>
<term>Test ELISA (normes)</term>
<term>Zoonoses (diagnostic)</term>
<term>Zoonoses (immunologie)</term>
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</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes viraux</term>
<term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps monoclonaux</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term>
<term>Zoonoses</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Zoonoses</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Infections à coronavirus</term>
<term>Zoonoses</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Middle East Respiratory Syndrome Coronavirus</term>
<term>Zoonoses</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Test ELISA</term>
</keywords>
<keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Camelus</term>
<term>Disease Reservoirs</term>
<term>Female</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Chameaux</term>
<term>Femelle</term>
<term>Humains</term>
<term>Réservoirs d'agents pathogènes</term>
<term>Sensibilité et spécificité</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Test ELISA</term>
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<front><div type="abstract" xml:lang="en"><p>Since its emergence in 2012, more than 900 laboratory-confirmed cases of Middle East respiratory syndrome (MERS) have been reported with a fatality rate of more than 30%. However, no antigen detection assay for commercial use is available for diagnosis. In this study, the full-length nucleocapsid protein (NP) gene of MERS coronavirus (MERS-CoV) was cloned and expressed in <italic>Escherichia coli</italic>
. A MERS-CoV NP capture enzyme-linked immunosorbent assay (ELISA) using two MERS-CoV-NP-specific monoclonal antibodies (MAbs) generated was developed. The ELISA was evaluated using 129 nasopharyngeal aspirates (NPAs) positive for various respiratory viruses and simulated positive NPAs by adding serial dilutions of MERS-CoV. Using a cutoff OD of 0.19, all 129 NPAs positive for respiratory viruses showed very low OD, with a specificity of 100%. For the two simulated MERS-CoV-positive NPAs with serial dilutions of live MERS-CoV, all samples with ≥10 50% tissue culture infective dose (TCID<sub>50</sub>
)/0.1 mL showed positive results. For the 10 additional NPAs with 20 and 200 TCID<sub>50</sub>
/0.1 mL of live MERS-CoV added, all were positive. A highly sensitive and specific MAbs-based antigen capture ELISA has been developed for MERS. This sensitive and specific antigen capture ELISA should be useful for detection of MERS-CoV in human and dromedaries and in field studies.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chan, Jf" uniqKey="Chan J">JF Chan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Groot, Rj" uniqKey="De Groot R">RJ de Groot</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reusken, Cb" uniqKey="Reusken C">CB Reusken</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Haagmans, Bl" uniqKey="Haagmans B">BL Haagmans</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Briese, T" uniqKey="Briese T">T Briese</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hemida, Mg" uniqKey="Hemida M">MG Hemida</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bermingham, A" uniqKey="Bermingham A">A Bermingham</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Guery, B" uniqKey="Guery B">B Guery</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lu, X" uniqKey="Lu X">X Lu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chan, Jf" uniqKey="Chan J">JF Chan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gui, X" uniqKey="Gui X">X Gui</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gui, X" uniqKey="Gui X">X Gui</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yuan, Q" uniqKey="Yuan Q">Q Yuan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kapoor, M" uniqKey="Kapoor M">M Kapoor</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reusken, Cb" uniqKey="Reusken C">CB Reusken</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meyer, B" uniqKey="Meyer B">B Meyer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Azhar, Ei" uniqKey="Azhar E">EI Azhar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chu, Dk" uniqKey="Chu D">DK Chu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nowotny, N" uniqKey="Nowotny N">N Nowotny</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Alagaili, An" uniqKey="Alagaili A">AN Alagaili</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
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</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name>
</noRegion>
<name sortKey="Chan, Jasper Fw" sort="Chan, Jasper Fw" uniqKey="Chan J" first="Jasper Fw" last="Chan">Jasper Fw Chan</name>
<name sortKey="Chan, Jasper Fw" sort="Chan, Jasper Fw" uniqKey="Chan J" first="Jasper Fw" last="Chan">Jasper Fw Chan</name>
<name sortKey="Chan, Jasper Fw" sort="Chan, Jasper Fw" uniqKey="Chan J" first="Jasper Fw" last="Chan">Jasper Fw Chan</name>
<name sortKey="Chan, Jasper Fw" sort="Chan, Jasper Fw" uniqKey="Chan J" first="Jasper Fw" last="Chan">Jasper Fw Chan</name>
<name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name>
<name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name>
<name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name>
<name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name>
<name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name>
<name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name>
<name sortKey="Kang, Yahong" sort="Kang, Yahong" uniqKey="Kang Y" first="Yahong" last="Kang">Yahong Kang</name>
<name sortKey="Kang, Yahong" sort="Kang, Yahong" uniqKey="Kang Y" first="Yahong" last="Kang">Yahong Kang</name>
<name sortKey="Lau, Susanna Kp" sort="Lau, Susanna Kp" uniqKey="Lau S" first="Susanna Kp" last="Lau">Susanna Kp Lau</name>
<name sortKey="Lau, Susanna Kp" sort="Lau, Susanna Kp" uniqKey="Lau S" first="Susanna Kp" last="Lau">Susanna Kp Lau</name>
<name sortKey="Lau, Susanna Kp" sort="Lau, Susanna Kp" uniqKey="Lau S" first="Susanna Kp" last="Lau">Susanna Kp Lau</name>
<name sortKey="Lau, Susanna Kp" sort="Lau, Susanna Kp" uniqKey="Lau S" first="Susanna Kp" last="Lau">Susanna Kp Lau</name>
<name sortKey="Luk, Hayes Kh" sort="Luk, Hayes Kh" uniqKey="Luk H" first="Hayes Kh" last="Luk">Hayes Kh Luk</name>
<name sortKey="Poon, Rosana Ws" sort="Poon, Rosana Ws" uniqKey="Poon R" first="Rosana Ws" last="Poon">Rosana Ws Poon</name>
<name sortKey="Woo, Patrick Cy" sort="Woo, Patrick Cy" uniqKey="Woo P" first="Patrick Cy" last="Woo">Patrick Cy Woo</name>
<name sortKey="Woo, Patrick Cy" sort="Woo, Patrick Cy" uniqKey="Woo P" first="Patrick Cy" last="Woo">Patrick Cy Woo</name>
<name sortKey="Woo, Patrick Cy" sort="Woo, Patrick Cy" uniqKey="Woo P" first="Patrick Cy" last="Woo">Patrick Cy Woo</name>
<name sortKey="Woo, Patrick Cy" sort="Woo, Patrick Cy" uniqKey="Woo P" first="Patrick Cy" last="Woo">Patrick Cy Woo</name>
<name sortKey="Xia, Ningshao" sort="Xia, Ningshao" uniqKey="Xia N" first="Ningshao" last="Xia">Ningshao Xia</name>
<name sortKey="Xia, Ningshao" sort="Xia, Ningshao" uniqKey="Xia N" first="Ningshao" last="Xia">Ningshao Xia</name>
<name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
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Serveur d'exploration MERS

A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus

A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus

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	Serveur d'exploration MERS
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